I have been plating some stuff for practice and fun, trying to work out some of my problems. I think I am leaving the plates out too long at room temperature before streaking them, so they are drying out. You can see the cracks, which are not necessarily where I streaked. I still have a bit of a heavy hand with the needle while streaking so those tend to break the agar in a few days, too. I am usually only making 100ml of media so it’s hard to measure the agar consistently with my regular scale. I need a jeweler’s scale, and perhaps lab grade agar powder instead of the flakes I bought at Whole Foods. This latest batch of media was made with bakers’ type malt extract since I had run out of the brewing stuff. It is very dark and probably not all that great for this. It seems to be giving a hue to the colonies themselves (upper left on the tej plate; is that possible?) in some places. The media is just extract, Wyeast brewing nutrient and agar.
I had a bottle of Schneider Weisse, which is supposed to be bottle conditioned with the fermentation strain. Some searching showed it’s not the easiest to culture, probably due to age and harsh shipping conditions from Europe. But with some careful sanitation I diluted the dregs in sterile wort and streaked a few days later. I see only yeast on the plate. It was quite slow to grow but it did. I haven’t tried to culture it in liquid.
As mentioned in the Tej post I streaked out the yeast during active fermentation. I saw only yeast at first though after looking at this picture I see what looks to be some type of bacteria colony right in the middle. There is some tang to the tej so I was actually looking for some LAB. I’m still working out how to take close-up shots without too much reflection, so pardon the quality. The cracks in the agar don’t help. There are spots on the outside that kind of look like they’re inside, but they are just hard water stains.